CRISPR Principles and Application
The course will cover the basics of genome editing while focusing on CRISPR/Cas9. The mechanisms behind genome editing tools will be explained, including newly discovered CRISPR variants. The participants will be able to clearly understand the steps involved in genome editing and the course will provide practical insights for successful gene editing.
- Emphasis on designing target site using open-source algorithms like CHOP-CHOP
- Generating single guide RNA (sgRNA) and other reagents like repair template for plasmid insertions and homologous recombination.
- Delivering CRISPR components to the cell
- Mutation Screening
- Phenotype Validation
- discussion on applications of CRISPR in R&D
- special emphasis on latest breakthroughs in treating genetic disorders using CRISPR
Completing this course will help you
Who is the course for?
This course tailored for faculty members teaching, Undergraduate and Postgraduate programs, Research Scholars and Scientists.
Types of GE tools, background on discovery, key applications Principle of CRISPR/Cas.
Identifying targets, different mutation types (frame shift, deletion), verify mutation by various techniques (e.g., PCR based methods, T7Endonuclease assay)
Making CRISPR reagents (sgRNA, Plasmids) Design repair template to insert GFP Project work details
In vivo and ex vivo therapies, ongoing clinical trials, and results of pioneering studies.
General discussion, doubt clearing session and future directions of the course.